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KMID : 1377020220190030505
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Tissue Engineering and Regenerative Medicine
2022 Volume.19 No. 3 p.505 ~ p.523
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Heparin Immobilization of Tissue Engineered Xenogeneic Small Diameter Arterial Scaffold Improve Endothelialization
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Tardalkar Kishor
Marsale Tejesh
Bhamare Nilesh
Kshersagar Jeevitaa
Chaudhari Leena
Joshi Meghnad G.
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Abstract
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Background: Autologous vessels graft (Inner diameter?
Methods: Decellularization of graft was done using SDS, combination of 0.5% sodium dodecyl sulfate and 0.5% sodium deoxycholate and only sodium deoxycholate. Decellularization was confirmed on basis of histology, and DAPI. Characterization of extracellular matrix was analyzed using histology and scanning electron microscopy. Surface modification of decellularized vascular graft was done with heparin coating. Heparin immobilization was evaluated by toluidine blue stain. Heparin-coated graft was transplanted end to end anastomosis in femoral artery in rat.
Results: Combination of 0.5% sodium dodecyl sulfate and 0.5% Sodium deoxycholate showed complete removal of xenogeneic cells. The heparin coating on luminal surface showed anti-thrombogenicity and endothelialization. Mechanical testing revealed no significant differences in strain characteristics and modulus between native tissues, decellularized scaffolds and transplanted scaffold. Collectively, this study proposed a heparin-immobilized ECM coating to surface modification offering functionalize biomaterials for developing small-diameter vascular grafts.
Conclusion: We conclude that xenogeneic decellularized arterial scaffold with heparin surface modification can be fabricated and successfully transplanted small diameter (inner diameter?
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KEYWORD
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Xenogeneic, Vascular graft, Decellularization, Small diameter vessels, Heparin surface modification
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